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(17) (17) Preparation of crude product Gly-Glu(otBu)-Pro-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin(TFA9.5mlH 2 O0.5ml)1.5500ml35003324 BPC1571.23gHPLC78 Pour the dried Gly-Glu(otBu)-Pro-Pro-Pro-Gly-Lys(Boc)-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin into the glass Add pre-cooled peptide cutting reagent (TFA9.5ml, H 2 O0.5ml) to the cutting bottle, react at room temperature for 1.5 hours, filter to remove the resin, add 500ml of glacial ether to precipitate, centrifuge at 3500 for 3 minutes to collect the precipitate, and diethyl ether After washing 3 times, dissolving with pure water, and lyophilizing at low temperature for 24 hours, 1.23 g of crude product of BPC157 peptide was obtained, and the purity was 78% as determined by HPLC

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[DOI] [PubMed] [Google Scholar] 116.Chan C.K., Tian F., Pimentel Maldonado D., Mowry E.M., Fitzgerald K.C

Immune escape mechanisms as a guide for cancer immunotherapy

At the end of this process, it is possible to seperate mRNA usually by EDTA, then by means of reverse transcription, DNA are attained and amplified [9]