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While you can use sterile water for a single, immediate use where the entire vial is consumed, we strongly advise against it for multi-dose protocols

Notably, inflammatory conditions may lead to a positive feedback loop in microglia, resulting in the secretion of proinflammatory mediators (Kuno et al., 2005), which can be neurotoxic and worsen existing damage (Zhang et al., 2004

Physicochemical properties and stability of black cumin (Nigella sativa) seed oil as affected by different extraction methods

Das B, Dadhich P, Pal P, Dhara S (2016) Single step synthesized sulfur and nitrogen doped carbon nanodots from whey protein: nanoprobes for long term cell tracking crossing the barrier of photo-toxicity

Agronomy 12 (3), 731 (2022)

Concomitant memantine and Lactobacillus plantarum treatment attenuates cognitive impairments in APP/PS1 mice