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The isolation buffers were collected and centrifuged (400 g, 4 min, 4 C)
suis , which only transiently infects humans and would therefore require repeated administration, N

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After incubation for 15 min in a 32 C water bath and 1 h at ambient temperature, the slices were loaded with Fura-2-AM (4 mM

In conclusion, gliotoxin can induce cytotoxic effects with relatively high concentrations (more than 100 nM)

7C), demonstrating a DNA hydroxymethylation dependent regulatory effect of DMF
