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Moreover, nanobodies consist of only one Ig domain and thus are easier to engineer into multivalent formats than conventional antibodies 25,26

Consequently, the GSH deprotonation step was considered non-rate-determining and thermodynamically favoured, leading us to use the GSH-thiolate as the starting point to compute the reaction mechanism for the conversion of flav-3-on-4-ol ( 7 ) into cyanidin ( 8-B4 ) in the wild-type enzyme and the C13S variant (Extended Data Fig

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This competitive binding may offer advantages over covalent inhibition by enhancing specificity and reducing the risk of non-specific interactions, which can limit covalent inhibitors therapeutic use