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These cytokines can signal the central nervous system to induce fatigue and sickness behavior, a pathway analogous to mechanisms seen in chronic inflammatory and infectious conditions [10]

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RNA isolation and quantitative RT-PCR gene expression levels The total RNA from the kidney tissue was isolated using the TRIzol reagent (Invitrogen) [27], and the cDNA was synthesized following the manufacturers protocol, using 1 g RNA (Prime ScriptTM RT reagent Kit, Takara)