glutathione s transferase protein purification Glutathione-S-Transferase (GST)-Fusion Based Assays for Studying Protein- Interactions GST-tagged Protein Purification Kit

LIP: labile iron pool

SCY, J-IC, and SP contributed to the clinical interpretation of the findings

Brodkorb A, Egger L, Alminger M, Alvito P, Assuno R, Ballance S, et al

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In the coagulation cascade, a key step is the conversion of prothrombin into the active form by carboxylation of glutamic acid to -carboxy-glutamate

Peptide digests were cleaned by solid phase extraction (SPE) using Strata-X 33 m polymeric sorbent cartridges (Phenomenex, 30 mg/1 mL, #8B-S100-TAK), as per the manufacturers instructions, with final elutes using sequential addition 70% MS-grade acetonitrile (ACN) with 0.5% FA and 90% MS-grade ACN with 0.5% FA