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We further administered LY294002 and L-NAME to rat models of GC-induced ONFH to elucidate the angiogenic effects of GDF11 in vivo, PI3K-antagonist group was treated with 0.5 mg/kg LY294002 via intraperitoneal (i.p.) injection, eNOS-antagonist group was treated with 20 mg/kg L-NAME, groups were administered daily for 3 consecutive weeks from the second day after MP injection