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-Oxidation of these compounds precedes their entry into the Krebs cycle, where energy production occurs

Protein in sample A and B was removed by precipitating with 200 L of cold ACN (containing 1 g/mL carbachol, the internal standard, IS) and centrifuging at 16,900 g for 20 min

The synthesis conditions used were as follows: an initial cycle at 50 C for 2 min, one cycle of polymerase activation at 95 C for 10 min, followed by 40 cycles with a denaturation temperature at 95 C for 15 s, an alignment of 60 C by 60 s and an extension at 72 C by 45 s

A., Demenice R., et al

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