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For NMR measurements, the samples were re-suspended in 200 L NMR buffer (50 mM sodium phosphate buffer in D 2 O at pH 7.2 with 0.1 mM DSS (4,4-dimethyl-4-silapentane-1-sulfonic acid) for referencing)

M.AltansuvdJ

Furthermore, increases in cellular GSH levels by either supplementing with the thiol-donor NAC or overexpressing GSH synthetic enzyme GCLC or/and GCLM subunit(s) diminished cellular ROS levels and reduced cellular 2GSH/GSSG redox potential by 712 mV (117)

PMID 8669840

MPGES1 have a key role in inflammatory diseases and is high upregulated in tissues during inflammation and overexpressed in tumors 23