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The role and properties of the iron-sulfur cluster in Escherichia coli dihydroxy-acid dehydratase

doi: 10.1038/nature05383

Studies have found that copper can induce neuronal cell ferroptosis, and FeCl2 and CuCl2 can both deplete GSH in mouse hippocampal neurons while enhancing ferroptosis induced by erastin, sulfasalazine (SSZ), and buthionine sulfoximine (BSO)

M.DhananiM.FannonM

After acclimatized for a week, the mice were randomly divided into different groups ( n = 6 per group) as follows: NOR (normal, untreated), CON (control, 0.9% saline with ethanol), Cys (200 mg/kg Cys with ethanol), GSH (200 mg/kg GSH with ethanol), MIX (200 mg/kg mixture of Cys and GSH with ethanol)

The following day, membranes were washed three times with TBS-T, and incubated with the secondary antibodies 680RD goat anti-rabbit IgG (Licor, 926-68071