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[PubMed] [Google Scholar] 19.Lynch SR, Cook JD

Key features Simple and fast protocoluser-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period Broad range of kitsacross key analytes and targets within the most important metabolic pathways Consistentstrict quality control criteria to ensure reproducible and reliable results

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Lysates were combined with 6 Laemmli SDS sample buffer (Boston BioProducts, BP-111R) containing 5% 2-mercaptoethanol (VWR Life Science, M131), then loaded onto 420% Criterion TGX pre-cast gels (Bio-Rad, 5671093)

Keep each peptide in its own vial, draw with its own syringe, and inject at separate sites