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Approximately 500 g of the mitochondrial pellet was resuspended in 200 L of nondenatured lysis buffer (WA-010, Invent Biotechnologies), a mild formulation designed to preserve native protein-protein interactions within the mitochondria while solubilizing mitochondrial membranes

doi: 10.3390/pharmaceutics11050239

Kran T, Otlu O, Karabulut A

Amitripeptide is not easily oxidized in the air

Moreover, the mitochondrial calcium uniporter (MCU) increases mitochondrial ROS (mROS), activating the Keap1-Nrf2 antioxidant response and upregulating SLC7A11, which enhances cystine uptake and inhibits ferroptosis in PDAC [113]