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Total RNA of blood stages obtained from mice with asynchronous infections was isolated using RNA STAT-60 (Tel-Test Inc.) according to manufacturer's specifications
Protein extracts were separated on 8% SDS-polyacrylamide or 412% bistris protein gel (Invitrogen), transferred on a nitrocellulose membrane, and stained with antibodies against ZO-1 (N1N2, GeneTex, dilution 1:3000)
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doi: 10.1016/0090-6980(81)90123-4
Alleviated diabetes-induced mesangial expansion, focal interstitial inflammation, glomerular atrophy, and glomerular sclerosis
Global epidemiology of diabetic foot ulceration: a systematic review and meta-analysis
