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It is also important for a precise detection of disease condition as mis-information or incorrect diagnosis will lead to wrong treatment plans that will in turn lead to the worsening of the disease or may result in delayed response in the patient (Wang and Wang, 2023)

Common risk factors for DED include aging, hormonal changes, autoimmune diseases, environmental factors, and certain medications [53]

Fluoro-Jade C staining for degenerating neurons Tissue processing Frozen whole brains, excluding cerebellum, were cryosectioned either by the Histology Core at the University of Colorado Anschutz medical campus or AML Laboratories Inc

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