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This doubling in the population of NSC upon 4D overexpression was essentially identical to that previously described in both neurogenic niches of young mice 25,30 and consistent with the recent observation that activated NSC of the young and old brain react similarly to proliferative stimuli 33

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Given the nature of syringes and needles, the procedure can be quite delicate and potentially painful

A fresh syringe per draw is the documented standard