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If GSH is depleted due to oxidative stress, inflammation, or exposure to xenobiotics, de novo synthesis of GSH is upregulated primarily by increasing availability of cysteine through recycling of GSSG

Kang YA, Choi HR, Na JI, Huh CH, Kim MJ, Youn SW, Kim KH, Park KC

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It also plays a role in maintaining skin elasticity, making skin look firmer and healthier

The key variables are the volume of water added (which determines concentration), proper sterile technique (which prevents contamination), and correct unit math (which ensures accurate dosing)