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The total protein concentration was then determined using BCA Protein Colorimetric Assay Kit (E-BC-K318-M, Elabscience, China), and the samples were boiled at 100 C for 5 min

169,170,171 The activation of LOX/LOXL stimulates signaling pathways involving protein kinase C and protein tyrosine kinase 2, further driving the activation of transcription factors associated with cancer cell proliferation and metastasis

doi: 10.1016/j.ejmg.2012.09.003

After blocking in 5% non-fat milk, the membranes were incubated overnight at 4 C with primary antibodies

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Gttng too little lum n increase the munt f xlt tht gets t th kidneys, whh wll nr the rk f kidney tn
