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We utilized Superscript III reverse transcriptase enzyme (Invitrogen) with random hexamer primers and 3 g of RNA from each sample to produce cDNA according to the manufacturers protocol

Each GLRX wild type or mutant has two 400-ns simulation replicas while each GLRX multimer was simulated with two replicas, 200 ns each (Supplementary Table 3)

This section delves into the mechanisms by which gut microbiota perturbations influence host inflammatory responses and immune regulation, contributing to the pathogenesis of hypothyroidism (Figure 3)

sapidus were transferred into 250-mL shake flasks containing 95 mL of a co-culture medium and cultured for 5 days

By restoring immune tolerance, halting fibrotic progression, and repairing multisystem damage, these advanced protocols provide new hope for patients burdened by MCTD

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