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doi: 10.1007/s00299-021-02691-8
3.2.4.2 T cell In the tumor microenvironment, T cells regulate tumor cell ferroptosis through multidimensional molecular mechanisms

Registration number of the article: R000025857, UMIN: UMIN000022552

Crockford D, et al

Acetyl-L-Carnitine Hydrochloride

Prokaryotic expression, purification and confirmation of rIFN For the recombinant IFN- protein (rIFN) induced expression, overnight cultured pIFN-containing bacteria (rBL21) were added to LB medium (containing 100 g/mL Amp) at a ratio of 1:100, incubated at 37 C to OD 600nm 0.6 with the rotate speed of 150 r/min, then 0.1 mmol/L IPTG was added, and continue to cultivate at 16 C for 4 h
