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Early AMD patients have high levels of oxidized glutathione (glutathione disulfide), a biomarker for AMD

Hsu et al

High neuronal synaptic Zn concentrations (up to 300 M) are present transiently in specific areas in rodent CNS (such as the hippocampal mossy fibers) after neuronal hyperexcitation with kainic acid (KA), a process that is calcium mediated and might have a role in physiologic neuronal excitation (267, 274)

Poornima for technical support

If daily injection compliance is unreliable, the benefits will be inconsistent and the cost may not justify the results

Cells were fixed using 10% buffered formalin for 10 minutes, then permeabilized in blocking buffer (10% serum, 1% Triton X-100, in 1X PBS) for 1 hour, then primary antibody diluted in blocking buffer was applied overnight (See supplemental table 5 for antibody concentrations)