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TMZ (Sigma-Aldrich) was dissolved in DMSO and added to cells at a final concentration of 100 M as previously described ( PGLS Expression and Activity PGLS expression in tissue samples was assessed by quantitative RT-PCR using a SYBR Green assay kit with the following primers: forward primer (TGTGGCAACTGGAGAAGGCAAG), reverse primer (CTCGTCCAAGAACCAGCACAGT)

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