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Clinical wound healing studies involving direct application to open wounds have shown good tolerability without significant adverse effects, and wound healing outcomes are generally improved rather than impaired

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The fresh leaf samples were centrifuged at 11500 g for 15 min at 4 C and the 0.4 mL of the supernatant was then added to 1 mL of 0.5 M potassium phosphate buffered at pH 7.5

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