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After centrifugation (0.5 g ), beads were resuspended in 600 l lysis buffer containing 10 mM NEM and split to input, minus hydroxylamine (HAM) and plus (+HAM) conditions before washing with lysis buffer containing 0.1% SDS

From a translational perspective, the question remains if and how the intradiscal implantation of MSCs (whether or not delivered using a carrier) in chronic degenerated discs will result in the inhibition of neurite ingrowth into the disc and pain relief

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Although some uncharged and lipid-soluble molecules can pass through the surface layer, it is unlikely that nutrients delivered via topical application would easily penetrate into the lower layers of the dermis [22]

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