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The next day, samples were washed three times with RIPA buffer containing quenching components, followed by three washes in 3 M Urea buffer (prepared with 50 mM NH 4 HCO 3 solution)

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HSS orthopedic surgeons and colleagues in other areas of medicine at Weill Cornell Medicine and Memorial Sloan Kettering Cancer Center are affiliated with and collaborate at the Neurofibromatosis Surgery Program at NewYork-Presbyterian Hospital/Weill Cornell Medical Center