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The next day, cells were collected in ice cold PBS, pelleted and sonicated in NP-40 lysis buffer (50 mM Tris pH 8, 150 mM NaCl, 5 mM EDTA, 0.5% NP-40) with protease inhibitors (Roche)

doi: 10.1111/j.1396-0296.2004.04019.x

[13] [15] Rare but serious side effects include hypersensitivity reactions, such as urticaria, angioedema, or anaphylaxis, which require immediate medical attention

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[DOI] [PMC free article] [PubMed] [Google Scholar] 146.Pakravan K, Mossahebi-Mohammadi M, Ghazimoradi MH, Cho WC, Sadeghizadeh M, Babashah S

In other molecular and neurochemical assays combined with behavioral evaluations, it was found that NAC effectively reversed a drop in the accumbal x c system and GLT-1, seen 7 or 50 days after cue-induced reinstatement, respectively [39, 40]