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Given these, structure-based rational design was conducted: (1) Engineering of the substrate-binding region of Pp PETase (Y250N mutation) improved the degradation of long-chain PET by 2.1-fold, which might be caused by the enhanced mobility of the extended loop and facilitating the accommodation of long-chain PET structures

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Citrate can be exported from the mitochondria to the cytosol to inhibit phosphofructokinase, another key enzyme in the control of glycolysis

[DOI] [PMC free article] [PubMed] [Google Scholar] 99.Tasian GE et al