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2.3 Laboratory-scale glutathione affinity chromatography Unless otherwise indicated, laboratory-scale GSH affinity chromatography was performed with GSTPrep FF 16/10 columns packed with GSH Sepharose 4 FF resin (Cat#: 28936550, Cytiva) run at a 6 min residence time using an AKTA Pure 150 M FPLC system (Cytiva) with UNICORN system control software (Cytiva)

By delivering glutathione directly into the bloodstream, the IV drip ensures that higher concentrations of this vital antioxidant are available for detoxification

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However, the precise mechanisms by which SCFAs influence brain physiology and behavior remain to be fully clarified, underscoring a promising avenue for developing novel CNS therapies

Once the peptides are reconstituted (mixed with bacteriostatic water), they must be stored in the fridge to maintain stability

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