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Each washed cell pellet was ultimately suspended in an equal volume of carbon- and nitrogen-free minimal media, and the resulting cell suspensions were used at 1% (vol/vol), i.e., 2.0 L, for the inoculation of 0.2 mL of test medium housed in a 96-well flat-bottom polystyrene plate (Falcon)

Research has shown significant interest in its potential role in tissue repair across multiple organ systems

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F., McKenney, J., Bloedon, L

From the article: We have identified a specific signal that is generated in visceral fat, released into the blood that gets through the blood brain barrier and into the brain where it activates microglia and impairs cognition. Visceral fat as the ring leader: They looked further and found that just transplanting the visceral fat caused essentially the same impact as obesity resulting from a high-fat diet, including significantly increasing brain levels of interleukin-1 beta and activating microglia