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Peripheral blood T cell responses to BPC were tested by means of the method described by deSmet et al (deSmet MD et ai

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coli lacZ ORFs via fusion PCR [13], and the resulting aatJ - lacZ and aatQ - lacZ fusions were gel purified and subsequently cloned into pCR-Blunt (Invitrogen) to yield pJMS07 and pJMS08, respectively