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Consistent inhibition of S6K1 activity in peripheral blood mononuclear cells was seen with doses 20 mg per week and was sustained for at least 7 days at doses 20 mg per week (O'Donnell et al, 2008)

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These samples were kept at 70C using a Termomixer (Eppendorf, City, Germany) for 24 h

4-HNE also regulates the Nrf2 pathway by binding to Keap1, its cytosolic inhibitor, promoting its release and allowing Nrf2 nuclear translocation

Sulforaphane, an activator of Nrf2, suppresses cellular accumulation of arsenic and its cytotoxicity in primary mouse hepatocytes