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Both chemicals were dissolved in phosphate-buffered saline (PBS)

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Proteomic analyses Pellets from IECs were resuspended in 50 l 6 M guanidine hydrochloride 100 mM Tris pH 8.5 containing 1.5 mg/mL TCEP and 1 mg/mL chloroacetamide and digested with 1 g LysC (FUJIFILM Wako Pure Chemicals U.S.A

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