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In vitro studies confirm minimal cytotoxicity with no impact on cell viability up to 100 M concentrations across multiple cell types, though higher concentrations may produce non-specific effects that could confound experimental results

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These findings suggest that NNMT inhibition may influence tumor cell line phenotypes in vitro through both metabolic and epigenetic mechanisms, making 5-amino-1MQ a valuable tool for investigating the metabolic characteristics of different tumor cell lines

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