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IL-12 is a proinflammatory cytokine involved in the differentiation of naive CD4 T-cells to their Th1 subset, thus inducing the production of IFN- and IL-2
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RNC-NAC-NMT1 in vitro reconstitution for cryo-EM 100 nM RNCs were incubated with 135 nM wildtype NAC and 135 nM GST-NMT1 in Reconstitution Buffer (20 mM HEPES/KOH pH 7.5, 150 mM KOAc, 5 mM MgCl 2 , 1 mM DTT, 0.05% octaethylene glycol monododecyl ether, 100 mM sucrose) for 1 h on ice before directly preparing cryo-EM grids
