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Figure 3 3.4 Temporal coordination of innate and adaptive immune compartments in gut-associated lymphoid tissue over the course of demyelination and remyelination Given the observed alterations in GM composition, microbial metabolites, and mucus dynamics, we next investigated whether these changes were accompanied by shifts in intestinal immune compartments, focusing on colonic macrophage polarization and T cell populations within gut-associated lymphoid tissue (GALT) ( Figure 4 )

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Oxidative Stress Thirty minutes after ligation, oxidative stress in the collected tissue samples (plasma, ICV) was assessed by quantifying thiobarbituric acid reactive species (TBARS) as malondialdehyde (MDA) [5,6,38,39,40]