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Drug-metabolizing enzymes: mechanisms and functions

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Tissue Barriers 9 , 937013 (2021)

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5 l of hemolyzed blood was diluted in 1 ml of potassium phosphate buffer (0.05 M, pH 7.0) with EDTA 0.1 mM, and finally in 10 l of H 2 O 2 (1 M) according to the standard procedure of Beers and Sizer

Gene expression analysis using quantitative real-time PCR (qRT-PCR) showed a reduction of approximately 80 and 90%, respectively, in SlPDS and SlBEL11 transcripts in the yellow areas of the TRV- SlPDS and TRV- SlBEL11 fruits, compared with the red TRV-Control fruits (Figures 1B, C)