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DNA methylation interferes with transcription by preventing the basic transcription mechanism or the binding of ubiquitous transcription factors (TF), which require contact with cytosine (C) in the main groove of the double helix

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Post-treatment fluorescent labeling is achieved by the initial exposure of cancer cells to alkyne functionalized P-chirogenic Au(III) followed by the biorthogonal Cu(I)-catalyzed azidealkyne cycloaddition (CuAAC) reaction using an azide-tagged FITC fluorophore