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After incubation for 72 h, the supernatant was removed, and viability was assessed by the PrestoBlue assay (Life Technologies, Carlsbad, CA, USA), according to the manufacturers instructions

Additionally, the availability of standard substances facilitates accurate quantitative analysis by referencing their spectral information

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These results provide compelling evidence for the previously unrecognized role of VSMC-derived SIRT7 as a positive regulator in protecting against aneurysm formation
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Ischaemia can also be caused by the rupture of intracranial vessels, resulting in intracerebral haemorrhage
