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This has lead to the use of alternate velocity equations which do not use the kinetic constants kcat and Km at all, instead depending on a different rate constant, (Toppo et al., 2009)

To construct the Pool-100 plasmid pool, 100 colonies were isolated, dissolved in 80 l of LB medium containing 100 g ml 1 ampicillin, combined in 5 l aliquots and cultured overnight at 37 C

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