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A Randall-Selitto probe was applied to the left gastrocnemius muscle to generate an automatic increase in pressure, with the mechanical withdrawal threshold being measured when the rat withdrew its limb

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Yang S, Song Y, Hu Y, Chen H, Yang D, Song X

If subclinical vitamin B12 is suspected, then circulating total vitamin B12 or transcobalamin (TC)-bound B12 (i.e., holo TC) in serum should be measured first, followed by methylmalonic acid (MMA) or homocysteine in serum, both more sensitive functional biomarkers that reflect B12 stores rather than intake

To determine the H 2 O 2 content, the extract (0.5 mL) was mixed with potassium phosphate buffer (pH 7, 0.5 mL) and 1 M potassium iodide (1 mL) and incubated for 10 min