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Feature GST Pull-Down Immunoprecipitation Binding Moiety GST Tag Antibody (Protein A/G/L) Beads Glutathione Agarose Beads Protein A/G/L Agarose Elution Harshness Mild Harsh Specificity High Varies Experimental Requirement Cloning with GST Tag Antibody availability How to do a GST pull-down