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Clinical Psychology Review, 34(3), 249255

The sterile workflow was performed under a laminar flow (HeraSafe KS, Thermo Scientific) equipped with a vacuum pump (BVC 21, Vacuumbrand)

You've introduced a variablethe potential for contamination with every single use
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This resulted in 20 participants assigned to Sequence A and 20 participants assigned to Sequence B

For the preparation of medium samples for metabolomics, 10 l of media was collected and 990 l of medium extraction buffer (80% methanol, containing 2 M d27 myristic acid) was added
