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Following 24 h of exposure to CMs, 3 10 5 HUVE cells/per FACS tube were stained for 30 min at 4 C with the following PE- conjugated mabs: anti-human CXCR4, anti-human CXCR1 (REAA958), anti-human CXCR2 (REA208), anti-human CCR2 (REA264), all purchased by Miltenyi biotech
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Additionally, like GPLC, it also stimulates nitric oxide production
