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DAPI staining solution was added to stain the cell nuclei

Handling and Storage Recommendations To preserve the quality and reproducibility of GHKCu (100 mg): Keep vials sealed at 20C or colder, dry, and protected from light

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Its primary job is to neutralize free radicalsunstable molecules that can cause cellular stress if left unchecked

ROS measurements ROS production was assessed using a luminol- and isoluminol-dependent chemiluminescence assay to measure intracellular and extracellular ROS, respectively, as previously described 60

Haas and Panula, 2016)