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PMID: 11101503)

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The following wash and elution sequence (1 mL) was performed using 96-well filter plates with a 25-m polyethylene frit (Agilent, 204495): 1 methanol, 1 x acetonitrile, 1 1% (v/v) formic acid, 1 10 mM Tris-HCl pH 8.0, add lysate and beads, 2 x Tris HCl pH 8.0 65,66