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After phase separation with chloroform, RNA was precipitated with isopropanol, washed with 75% ethanol, air-dried, and dissolved in RNase-free water

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Understanding it helps explain both why FTPP works and why its mechanism has attracted interest from cancer researchers, metabolic scientists, and aging researchers

Additionally, DSIP appears to be regulated by glucocorticoids, and its signaling may interact with the MAPK cascade through homology with glucocorticoid-induced leucine zipper (GILZ), suggesting potential roles in cellular stress responses

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