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CYP2E1 overexpression cell line establishment Rat cardiomyocyte cell line H9c2(2-1) (1101RAT-PUMC000219) was purchased from Cell Resource Center, CAMS/PUMC (Beijing, China) and was cultivated in high-glucose DMEM (Gibco, C11995500BT, USA) containing 10% fetal bovine serum (Gibco, 11995065, USA), 100 U/ml penicillin, and 100 g/ml streptomycin (Gibco, 15070063, USA) at 37 C with 5% CO 2 and 95% air and passaged after trypsin (Solarbio, T1320, China) digestion

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The role of BBB permeability changes, inflammation and neuroinflammation, oxidative stress, ferroptosis, and the gut microbiome in perioperative BBB damage and molecular mechanisms need to be further explored