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10.1016/j.bone.2005.03.018

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We reference the COA to determine the verified concentration and purity of each product, regardless of what the label or product image indicates

For pH-dependence measurements, purified Cystector was diluted in assay buffer with varying proportions of NaH 2 PO 4 /Na 2 HPO 4 to reach pH 6, 6.4, 6.8, 7.2, 7.6 and 8

We also tested the possibility that Cys-Tyr thioether cofactor formation might alter the susceptibility of CDO to ubiquitination and proteasomal degradation by expressing wild-type and Cys93Ser mutant CDO in HepG2/C3A cells using a Tet-off system and Cys-supplemented medium