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Pairs of DEMs from the DEMs obtained from the LD muscles of Gayal versus yellow cattle demonstrated that 36 DEMs were up-regulated and 40 DEMs were down-regulated in the Gayal muscle in the positive ion mode, and 16 DEMs were up-regulated and 12 DEMs were down-regulated in the Gayal muscle in the negative ion mode (Supplementary Table S8)

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Eighty L of working reagent (containing 8 L substrate, 8 L co-substrate, 1 L GDH, 0.5 L DTNB and 70 L assay buffer per well) was added to each sample well and mixed