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Angiogenesis (London)

NAD+ is less stable in solution than many peptides, so prompt refrigeration matters

Anyone know if it would be ok

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The top layer was aspirated without perturbing the disk, additional MeOH (400 l) was added, and the proteins were pelleted (10,000 g, 10 min, 4C) and the pellet were resuspended in 300 L of 50 mM TEAB, containing 2 M urea by water bath sonication