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The His 6 -tag was cleaved off by PreScission protease and the resulting authentic 306 amino acid M pro (see Figure S1A in supplemental material) and C300S M pro were further purified by SEC using a HiLoad Superdex 200 pg column (GE Healthcare) in 20 mM Tris, pH 7.5, 150 mM NaCl, and 2 mM DTT
E-BC-K271-M, Elabscience Biotechnology Inc., Houston, TX, USA), which is based on the ABTS method, in which antioxidants in the plasma samples inhibit the oxidation of ABTS to its radical cation (ABTS+), leading to a decrease in absorbance at 734 nm
2023;133(5):e162315
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How to Incorporate Them into Your Routine Building a routine with these two nutrients is straightforward, but consistency is key
